Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 15;27(12):1875–1884. doi: 10.1091/mbc.E16-01-0069

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Laporte et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 2: — Telomere hyperclusters colocalize with the nuclear membrane. (A) Telomere hyperclusters (Sir2-GFP) display slower MSDs in WT quiescent cells (7 d, red line) than in proliferating G₁ cells (green line). Error bars are SEM. (B) Telomere hyperclusters colocalize with the nuclear membrane. WT quiescent cells (7 d) expressing Nup2-RFP (red) and Sir2-GFP (green, left panel) or Sir3-GFP (green, right panel). (C and D) Telomere hyperclusters are distributed nonhomogenously around the quiescent cell nucleus periphery. (C) WT quiescent cells (7 d) expressing Sir2-GFP (green) and Bim1-RFP (red), a protein localized along the nuclear microtubule bundle, were used to analyze telomere hypercluster localization. The mean number of detected Sir2-GFP foci per cell and the percentage of cells displaying nuclear microtubule bundle in the population are indicated. (D) Left, schematics showing edge-based detection of telomere hyperclusters and nuclear microtubule bundle position. Right, Sir2-GFP localization (top, blue dots) and the corresponding heat map (bottom) are shown. In the two panels, a sum of nuclear volume 3D projections using the nuclear microtubule bundle (green) as an oriented symmetry axis reference (the SPB been the origin, yellow arrow) is shown. Scale bars: 2 μm.