Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 15;27(12):1928–1937. doi: 10.1091/mbc.E16-03-0143

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Razafsky et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 2: — Inactivation of Lmnb1 during embryonic development causes a collapse of the lamin B2 meshwork but does not prevent the birth of new postmitotic neurons. (A) Immunostaining of E14.5 ^Rx-CreLmnb1^Δ/Δ retinas with lamin B1 and lamin B2. In the merged image, lamin B1 is red and lamin B2 is green; DNA was stained with DAPI (blue). Bottom, zoomed-in views of the yellow boxed area in top. (B) Immunostaining of E14.5 ^Rx-CreLmnb1^Δ/Δ retinas with Lap2β and lamin B2. In the merged image, Lap2β is red and lamin B2 is green; DNA was stained with DAPI (blue). Bottom, zoomed-in views of the yellow boxed area in the top. The yellow arrow points to apoptotic nuclei in a gap. (C) Immunostaining of retinas from E14.5 ^Rx-CreLmnb1^Δ/Δ (top) and ^Rx-CreLmnb1^Δ/WT (bottom) mice with antibodies against lamin B2 and Brn3, a marker of RGCs. In the merged image, Brn3 is red and lamin B2 is green; DNA was stained with DAPI (blue). The arrows point to misshapen nuclei of RGCs. These studies show that RGCs devoid of lamin B1 are present and have irregularly shaped nuclei (yellow arrows). See also Supplemental Figure S2.