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. 2016 Jun 15;27(12):1928–1937. doi: 10.1091/mbc.E16-03-0143

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© 2016 Razafsky et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — B-type lamins are long-lived proteins that are dispensable for cone photoreceptor viability and function. (A) Top, Coimmunostaining of retinas from P42, P150, and P300 ^HRGP-CreLmnb1^Δ/ΔLmnb2^Δ/Δ (^HRGPLmnb1^Δ/Δb2^Δ/Δ) mice with antibodies against CAR and either lamin B1 (left) or lamin B2 (right). Yellow arrows point to cone photoreceptor nuclei labeled with CAR. Scale bars, 20 μm. Bottom, same experiment performed on retinas from P300 HRGP-Cre transgenic mice. (B) Coimmunostaining of retinas from 9-mo-old ^HRGP-CreLmnb1^Δ/ΔLmnb2^Δ/Δ and age-matched HRGP-Cre transgenic mice with antibodies against lamin A/C and CAR; these studies allowed us to count cone photoreceptor populations and assess cone photoreceptor morphology. The number of cones photoreceptors was determined in at least three fields of two retinas from two 9-mo-old mice of the indicated genotype. Note the scattering of nuclei in cone photoreceptors in ^HRGP-CreLmnb1^Δ/ΔLmnb2^Δ/Δ retinas. (C) An absence of B-type lamins in cone photoreceptors does not inhibit cone photoreceptor function. ERGs were performed on each eye from 10-mo-old ^HRGP-CreLmnb1^Δ/ΔLmnb2^Δ/Δ (n = 12) and three age-matched HRGP-Cre transgenic mice (n = 6). (D) Lamin B1 immunostaining of retinas from P300 ^Rho-CreLmnb1^Δ/Δ mice (left) and ^Rho-CreLmnb1^Δ/WT (right) littermate mice. Note the significant but incomplete down-regulation of lamin B1 expression in P300 rod photoreceptors (arrowheads).