Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 15;27(12):1938–1947. doi: 10.1091/mbc.E16-02-0090

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Zhang and Schekman. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 1: — ProTGFα requires an auxiliary cytosolic factor for efficient COPII packaging. HeLa cells were transfected with plasmids expressing pro/HA-TGFα, and membranes were harvested the next day for use in in vitro budding reactions. (A) Quantitative infrared fluorescence immunoblot (LiCor) analysis of in vitro budding reaction. Rat liver cytosol was prepared from fresh or frozen rat livers as indicated. Sar1A H79G is a GTPase mutant that is a dominant-negative inhibitor of in vitro budding. (B) Immunoblot of various cytosol fractions and purified COPII samples. (C) LiCor analysis of in vitro budding reaction. (D, E) Immunoblots of in vitro budding reactions. Total, total cytosol extract; 30P, pellet fraction of cytosol after 30% ammonium sulfate precipitation; 30S, supernatant fraction of cytosol after 30% ammonium sulfate precipitation. Syn1, purified recombinant human syntenin-1. Asterisks indicate unrelated bands.