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. 2016 Jun 15;27(12):1938–1947. doi: 10.1091/mbc.E16-02-0090

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© 2016 Zhang and Schekman. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Expression of CNIH in CNIH KO HeLa cells partially rescues proTGFα budding phenotype. (A–C) In vitro budding reactions. CNIH was detected using immunoblotting, and pro/HA-TGFα was detected using LiCor. Membranes were prepared from wild-type HeLa cells expressing pro/HA-TGFα (A), CNIH KO HeLa cells expressing pro/HA-TGFα and CNIH induced by 1 ng/μl doxycycline (B), or CNIH KO HeLa cells expressing pro/HA-TGFα without doxycycline induction (C). Lanes 3–6 in A–C are quadruplicate budding reactions. (D) Quantification of proTGFα budding efficiency. Results are represented as mean ± SEM (***p < 0.001). (E) Immunoblot showing the induction of CNIH expression. Cells were treated with the indicated amounts of doxycycline (DOX) for 24 h, and then lysates were harvested for analysis.