FIGURE 5:

CNIH and cytosolic factor(s) required for proTGFα recruitment to the prebudding complex. (A) HeLa cells were transiently transfected with plasmids expressing pro/HA-TGFα wild type (WT), V160G mutant (VG), or HA-Vangl2 (Vang) as indicated, and immunoprecipitation was performed using anti-HA antibodies. (B–E) Immunoblots of COPII recruitment assays. Recruitment of pro/HA-TGFα was examined using membranes prepared from WT HeLa cells expressing proTGFα WT (B) or V160G (C) or using membranes prepared from CNIH KO HeLa cells expressing proTGFα WT (D). (E) Recruitment of Sec22 compared in HeLa WT or CNIH KO membranes.