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. 2016 Jun 8;11:2663–2673. doi: 10.2147/IJN.S104593

Figure 4.

Figure 4

The RAW264.7 cell apoptosis induced by PPT and TP detected by flow cytometric method and ROS method.

Notes: The effect of (A) PBS, (B) PP, (C) 100 nM TP, and (D) PPT in which the TP concentration equals to 100 nM on apoptosis of RAW264.7 cells by flow cytometry by using PI and Annexin V-FITC to stain the apoptosis cells. (E) The ROS level after treatment with PBS, PP, 100 nM TP, and PPT was detected by 2,7-dichlorodihydro fluorescent diacetate kit.

Abbreviations: PPT, γ-PGA-l-PAE-TP; γ-PGA, poly-γ-glutamic acid; l-PAE, l-phenylalanine ethylester; TP, triptolide; PBS, phosphate-buffered saline; PP, l-PAE-γ-PGA complex; ROS, reactive oxygen species; FITC, fluorescein isothiocyanate; PI, propidium iodide.