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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: J Biomech. 2016 Feb 9;49(5):668–677. doi: 10.1016/j.jbiomech.2016.01.023

Fig. 2.

Fig. 2

Experimental Flowcharts. A. Engineering of puzzle-shaped constructs (Section 2.1): Puzzle and Square constructs cultured individually for 28 days. This experiment was repeated independently (Studies 1 and 2). B. Assembly of puzzle-shaped constructs (Section 2.2): Day 49 Evaluation of: Puzzle constructs assembled on day 28; Fresh constructs assembled on Day 49; and Square construct controls. Puzzle and Square constructs stem from Studies 1 and 2; Fresh constructs stem from Study 1. All assemblies are 3×3. C. Integration techniques for assembled puzzle-shaped constructs (Section 2.3): Puzzle constructs assembled on day 28 evaluated at days 49 and 63 (Extended Puzzle); Fresh constructs assembled and evaluated on day 49; and Square construct controls evaluated on days 49 and 63 (Extended Square). All assemblies are 2×2. All constructs stem from Study 1.