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A-B. Cross-sectional histology of assembled 2×2 puzzle arrangement at D49: Safranin O/Fast Green for GAG visualization (A) and Picrosirius Red for collagen visualization (B). (Puzzle, Square, Extended Puzzle: n=1; Fresh: n=2). Puzzle, Square, and Fresh groups were evaluated on day 49, while the Extended Puzzle group was evaluated on day 63. Scale bars: 1 mm. * indicates gaps between or within (“female” portion) subunits, ^ indicates area inside the fluid-filled sac.
