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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Hum Mutat. 2016 Mar 21;37(7):653–660. doi: 10.1002/humu.22983

Figure 2.

Figure 2

Yeast complementation assay using the Δ alg1 deficient yeast strain. The alg1 deficient yeast strain was transformed with the indicated pYES2.1 construct expressing either human wild-type ALG1 or the indicated missense variants and allowed to grow for 96 hours at both permissive (26°C) and restrictive temperatures (37°C). Western blot analysis of carboxypeptidase Y (CPY) glycosylation was performed as previously described [Grubenmann et al., 2004]. Both CPY glycosylation and growth complementation were performed in triplicate with representative data presented.