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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: J Immunotoxicol. 2016 Apr 4;13(3):428–438. doi: 10.3109/1547691X.2015.1120829

Figure 6.

Figure 6

(A) Gating strategy used to assess lung DC. Cells were first gated based on forward scatter (FSC) and side-scatter (SSC) characteristics. Cells positive for MCH-II and CD11c were determined to be either dendritic cells (DC, SiglecF−) or macrophages (MØ, SiglecF+). (B) Total number of MHC-II+/CD11c+/SiglecF− DC in WT mice exposed to air or ozone (0.3 ppm) for 24, 48 or 72 hr. (C) Total number of MHC-II+/CD11c+/SiglecF− DC in WT and Flt3l−/− mice exposed to air or ozone (0.3 ppm) for 72 hr. Results shown are means ± SE of 7–8 mice/group. *p < 0.05 vs. air-exposed mice with same genotype; #p < 0.05 vs. WT mice with same exposure.