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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Osteoarthritis Cartilage. 2016 Feb 20;24(7):1274–1283. doi: 10.1016/j.joca.2016.02.003

Figure 3.

Figure 3

Binding of NFAT1 to the promoter region of its candidate target genes. (A) NFAT1 ChIP assay followed by qPCR quantification using chromatin prepared from the AC of 6- and 18-month-old (6m and 18m) mice. Normal mouse IgG was used as a baseline control. (B) Representative agarose gel image of the PCR products of Acan primers verifies the specificity of NFAT1 ChIP assay. The chromatin isolated from the AC of Nfat1−/− mice was used as a negative control. N = 3.