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Production of tPA-coated and poly(acrylic acid-co-maleic acid) (PAM)-bearing superparamagnetic iron oxide nanoparticles (tPA@PAM-SPIONs). Reaction A: SPIONs with covalently bound tPA after EDC/NHC induced activation of PAM-containing carboxyl groups: covalent tPA@PAM-SPIONs. Reaction B: SPIONs with adsorbed tPA on the PAM-coated surface: adsorbed tPA@PAM-SPIONs
