Fig. 5.

RT-PCR and real-time PCR showing Minpp1 mRNA expression during apoptosis. a MC3T3 cells were treated with increasing concentrations of etoposide (25–100 μM) for 24 h and changes in Minpp1 mRNA expression during apoptosis were determined by RT-PCR. Shown is a representative experiment out of three with triplicate samples for each treatment. GAPDH was used as an internal control in all these experiments. b Effect of etoposide (ETOP; 100 μM), wortmannin (WMN; 500 nM), and cisplatin (CDDP; 100 μM) were used to induce apoptosis in MC3T3-E1 cells for 24 h. RT-PCR was carried out to determine the changes in expression of Minpp1 mRNA during apoptosis. Shown is a representative experiment out of three with duplicate samples for each treatment. GAPDH was used as an internal control in all these experiments. c Quantitative analysis using real-time PCR was performed on MC3T3 cells to determine changes in expression of Minpp1 mRNA. Data shown is a fold increase calculated from three independent experiments. The bar indicates means ± S.E. *p < 0.01