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. 2016 Apr 10;594(12):3353–3370. doi: 10.1113/JP271858

Figure 3. Effect of CTNS knockdown‐induced lysosomal cystine accumulation on iNOS and NOX expression .

Figure 3

HK‐2 cells were transfected with CTNS siRNA pool (siCTNS) for 24 h. A separate batch of cells was transfected with a non‐targeting siRNA (siNT) pool (50 nm) as a negative control. iNOS and NOX expression were determined by Western blot 72 h after transfection. Representative blots for iNOS and NOX are shown (A). GAPDH was used as a loading control. Densitometry data for iNOS and NOX were normalized for GAPDH expression and are expressed as fold change relative (rel.) to siNT ± SD of three independent experiments (B). *Statistically significant difference at P < 0.05.