Table 3.

Gating parameters for WT and mutant human ClC‐1 channels expressed in Xenopus oocytes

Oocyte	P o V 1/2 (mV)	P o V 1/2 (mV)	P o f V 1/2 (mV)	P o f V 1/2 (mV)	P o s V 1/2 (mV)	P o s V 1/2 (mV)	P o s residual	P o s residual
treatment	0 min	10 min	0 min	10 min	0 min	10 min	(%) 0 min	(%) 10 min
Control (WT, n = 6)	−68 ± 4	−76 ± 5*	−98 ± 3	−104 ± 3	−44 ± 6	−42 ± 7	52 ± 4	55 ± 5*
P value	—	0.001	—	0.102	—	0.499	—	0.025
200 nm PMA (WT, n = 9)	−83 ± 5	−31 ± 5*	−110 ± 4	−88 ± 4*	−44 ± 2	−6 ± 2*	59 ± 4	33 ± 2*
P value	—	<0.0001	—	<0.0001	—	<0.0001	—	<0.0001
200 nm PMA (C277S, n = 9)	−74 ± 4	−68 ± 4*	−83 ± 4	−79 ± 3	−37 ± 4	−41 ± 2	81 ± 3	75 ± 3*
P value	—	0.032	—	0.116	—	0.372	—	0.010

n represents number of oocytes and values are presented as means ± SEM. *Values significantly different from 0 min under the same conditions.