Figure 1. Effect of conditional MYPT1 gene ablation on content of myosin regulatory proteins in ileal tissues .

The relative contents of proteins were measured by immunoblotting extracts of ileal smooth muscle tissues from MYPT1^SM+/+ and MYPT1^SM–/– mice. Representative immunoblots (A, C and E) and quantified results (B, D and F) are shown for MYPT1, MBS85 and PP1C (α, γ,δ), as well as RLC, MLCK, CPI‐17 and ROCK1 for ileal tissues from MYPT1^SM+/+ (open bars) and MYPT1^SM‐/‐ (solid bars) mice with GAPDH as a loading control. MLCP component proteins MYPT1 and MBS85 were normalized to the MYPT1 content in ileal tissues from MYPT1^SM+/+ mice. MYPT1 and PP1cδ contents were similar in ileal tissues with or without mucosa in both MYPT1^SM+/+ and MYPT1^SM–/– mice (data not shown). Data are presented as the mean ± SEM from ≥10 animals in each group. ***P < 0.001 compared to MYPT1^SM+/+.