Figure 6. MLCK and CPI‐17 are phosphorylated in MYPT1‐deficient ileal tissues .

A, representative blots for MLCK phosphorylation and (C) its quantification are shown in response to 90 mm KCl (top) and 6 μm carbachol (CCh) (bottom) in ileal tissues from MYPT1^SM+/+ (open circles) and MYPT1^SM–/− (solid circles) mice. MLCK phosphorylation was normalized to the response obtained with calyculin A (CA) with GAPDH as a loading control. Values are the mean ± SEM (n ≥ 10). ***P < 0.001 compared to MYPT1^SM+/+ strips at the same time. B, representative blots for CPI‐17 phosphorylation and (D) its quantification are shown in response to 90 mm KCl (top) and 6 μm CCh (bottom) ileum tissues from MYPT1^SM+/+ (open circle) and MYPT1^SM–/− (solid circle) mice. CPI‐17 phosphorylation normalized to the response obtained with PDBu. Values are the mean ± SEM (n ≥ 10). ***P < 0.001 compared to MYPT1^SM+/+ strips at the same time. The SEM bars may be smaller than the circles for the means.