Figure 8. The knockin mutation MYPT1 T853A has no effect on force development and RLC phosphorylation in ileal tissues from MYPT1^T853A knockin mice .

A, representative blots for MYPT1 phosphorylation in response to 6 μm carbachol (CCh) showing no MYPT1 T853 phosphorylation in tissues from homozygous MYPT1^T853A knockin mice. B, force development responses in ileal strips from MYPT1^SM+/+ (open circles) and MYPT1^T853A (grey circles) are shown for 90 mm KCl (top) or 6 μm carbachol (CCh) (bottom) treatments. Data are the mean ± SEM (n ≥ 20). The SEM bars may be smaller than the symbols for the means. C, quantified RLC (top) and CPI‐17 phosphorylation (bottom) responses are shown for ileal strips from MYPT1^SM+/+ (open bars) and MYPT1^T853A (grey bars) mice to treatment with 6 μm CCh. CPI‐17 phosphorylation normalized to the response obtained with PDBu. Data are the mean ± SEM (n ≥10 animals).