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. 2016 Mar 12;7:11–15. doi: 10.1016/j.ymgmr.2016.02.002

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — Rapid LC-ESI-MS/MS analysis of 7α12αC4 in CTX positive and unaffected newborn DBS The left panels are extracted ion chromatograms for MRM detection of QAO tagged 7α12αC4 and QAO-d₃ tagged 7α12αC4 internal standard in CTX positive DBS samples (black and red traces, respectively; QAO tagged 7α12αC4 is the peak eluting at 0.98 min). Panel A is the analysis of a Dutch newborn sample stored for 3 years, panels B and C are the analysis of newborn samples stored for 15 years and 30 years respectively (analysis of these DBS with a 6 min run time was described previously [14]). The right panels are extracted ion chromatograms for MRM detection of QAO tagged 7α12αC4 and QAO-d₃ tagged 7α12αC4 internal standard in representative unaffected newborn samples.