Table 1.
Accuracy and precision data for calculated 7α12α-dihydroxy-4-cholesten-3-one (QAO-d0 12α7αC4) concentrations in DBS.
| Internal standard used | Nominal conc. (ng/ml) 7α12αC4 |
Calculated conc. (ng/ml) 7α12αC4 |
Accuracy (%) |
RSD (%) |
Calculated conc. (ng/ml) 7α12αC4 |
Accuracy (%) |
RSD (%) |
|---|---|---|---|---|---|---|---|
| Within-run (n = 3) | Between-run (n = 8 over 5 days) | ||||||
| QAO-d3 7α12αC4-d0 | 50.0 | 60.7 | 121 | 9.0 | 55.8 | 112 | 9.9 |
| 100 | 92.9 | 93 | 6.8 | 88.1 | 88 | 9.3 | |
| 500 | 461 | 92 | 15.1 | 492 | 98 | 10.4 | |
| QAO-d0 7αC4-d7 | 50.0 | 53.2 | 106 | 7.2 | 57.8 | 116 | 8.6 |
| 100 | 97.5 | 98 | 4.0 | 90.6 | 91 | 9.5 | |
| 500 | 525 | 105 | 6.6 | 511 | 102 | 4.8 | |