Table 4.
Polymorphism of 55 SSR primer pairs in medicinal Chrysanthemum morifolium samples.
| Primer pair (ID) | Primer Sequences (5′-3′) | Repeat motif | Tm | Size | No. of loci | No. of Polymorphic loci | Polymorphic loci % | PIC | References |
|---|---|---|---|---|---|---|---|---|---|
| CMeSSR001 | F:ATTCTGTCACTCAAACACCAC | (CATC)8 | 55.3 | 251 | 26 | 26 | 100.00 | 0.978 | This study |
| R:GGTTCAAACGAGCTAAATTACA | |||||||||
| CMeSSR002 | F:CTCACCATTTTCAGACCATTAT | (ACA)7 | 55.4 | 251 | 26 | 26 | 100.00 | 0.976 | This study |
| R:ACACATCTTGTACCTCTTGGTT | |||||||||
| CMeSSR003 | F:CTTTTTCACACACACTCAACAT | (TAA)7 | 54.5 | 256 | 26 | 26 | 100.00 | 0.976 | This study |
| R:TTGGAGACGTTGTTGTAAAGTA | |||||||||
| CMeSSR004 | F:AAAATGTTAGGTGCAGGATTAC | (AAT)7 | 54.3 | 248 | 27 | 27 | 100.00 | 0.977 | This study |
| R:AAAAACCGTTCCAGATTACAC | |||||||||
| CMeSSR005 | F:AAAACCTTCACTAGATCACACC | (CAC)7 | 56.3 | 252 | 29 | 29 | 100.00 | 0.978 | This study |
| R:TTTCAGTATCTTGGACCAGTCT | |||||||||
| CMeSSR006 | F:ATTCTCTTAATTAGCCAGCAAG | (CAC)7 | 54.3 | 247 | 17 | 17 | 100.00 | 0.958 | This study |
| R;GTGAATCGTAAATTCAGTTGG | |||||||||
| CMeSSR007 | F:GTCCTCCTTCAAAGCAAA | (CTA)7 | 51.3 | 157 | 28 | 28 | 100.00 | 0.976 | This study |
| R:GACGATTAATTATTGGGTAATA | |||||||||
| CMeSSR009 | F:AGTGATGATGAATTGAAAGAGC | (AAT)8 | 56.4 | 258 | 18 | 17 | 94.44 | 0.959 | This study |
| R:CTCTCAAGTGTTGAAGGAACTC | |||||||||
| CMeSSR010 | F:CATTTTCTTCATGGTACTCACA | (CAC)7 | 56.4 | 169 | 27 | 27 | 100.00 | 0.978 | This study |
| R:GTGAGGATGGAAATCTAGTAGG | |||||||||
| CMeSSR011 | F:AGGACAACTCAACTGTTAGGAG | (CCA)7 | 57.2 | 255 | 20 | 19 | 95.00 | 0.964 | This study |
| R:GTTTCTCAACCTCTTCTTCATC | |||||||||
| CMeSSR012 | F:ATTCCCAACCTTCTTTAACC | (CA)11 | 54.1 | 254 | 29 | 29 | 100.00 | 0.979 | This study |
| R:AACTAAATCACCATCTCTTGCT | |||||||||
| CMeSSR013 | F:ATGAGAGGGAAATAGAAAGTGA | (GTAATA)3(TAA)4 | 56.4 | 219 | 26 | 26 | 100.00 | 0.973 | This study |
| R:TACTTGACGCTAACGGAGTAGT | |||||||||
| CMeSSR014 | F:CAAAACTTTCAACAGAGTCATC | (CAACAT)3(CAG)4 | 54.5 | 281 | 35 | 35 | 100.00 | 0.983 | This study |
| R:AGAAATAACGACTGGTCAGATT | |||||||||
| CMeSSR015 | F:TCTTGGTCAGCTTAATTACTCA | (TGG)7…(TGG)4(AGG)4 | 57.1 | 236 | 30 | 29 | 96.67 | 0.979 | This study |
| R:CATCACCTCCTCCTCCTC | |||||||||
| CMeSSR016 | F:GAATACTAAATGGGTGGAAGAA | (GGA)4(GGT)4 | 55.3 | 250 | 18 | 18 | 100.00 | 0.967 | This study |
| R:GCAAATAGATGTCCTTTAGGG | |||||||||
| CMeSSR017 | F:TCATGAAATCCGTGTATATGTC | (AC)5a(AC)6 | 54.5 | 229 | 30 | 30 | 100.00 | 0.980 | This study |
| R:ACCCTAATTCTCAAAATGAACC | |||||||||
| CMeSSR018 | F:ATCTACTATCCAAGCCATGAAC | (CAC)5…(GGT)10…(TGG)6 | 56.8 | 264 | 25 | 23 | 92.00 | 0.973 | This study |
| R:TATCCACCACCACCACCA | |||||||||
| gi298295865 | F:ACTCACTTGCCCCATTTGTC | (AACCCT)5 | 59.8 | 146 | 15 | 15 | 100.00 | 0.957 | Wang H. B. et al., 2013 |
| R:AGAGAAGCTCTCCAGGGACC | |||||||||
| gi298300528 | F:AGGGCATCGATAATCCATCA | (ATATC)4 | 56.8 | 135 | 12 | 12 | 100.00 | 0.938 | Wang H. B. et al., 2013 |
| R:AGATACGTGCCCATTTGAGG | |||||||||
| gi298295793 | F:ATAGAATTCCCCGACGACAA | (CCCTAT)4 | 56.8 | 111 | 15 | 14 | 93.33 | 0.948 | Wang H. B. et al., 2013 |
| R:GGCGGTTGAGATTGATAGGA | |||||||||
| gi298296818 | F:ATGTCCAGCTTGATGGGAAG | (GTG)7 | 58.8 | 210 | 24 | 23 | 95.83 | 0.977 | Wang H. B. et al., 2013 |
| R:GGCCCCTTGCAAATCCTC | |||||||||
| gi298298301 | F:CTTGACCGAAACACCGAAAT | (TTG)9 | 56.8 | 198 | 17 | 16 | 94.12 | 0.958 | Wang H. B. et al., 2013 |
| R:TGGCATCCTAGTTAGCAGCA | |||||||||
| gi298299323 | F:GCACATTTCCTTCATGGGTT | (ACA)9 | 57.8 | 264 | 33 | 33 | 100.00 | 0.982 | Wang H. B. et al., 2013 |
| R:TCCACGGTTTCAGATGATGA | |||||||||
| gi298297301 | F:TCAAACACCACCACCAACAC | (CATC)8 | 58.8 | 167 | 25 | 25 | 100.00 | 0.973 | Wang H. B. et al., 2013 |
| R:ATGTCACCAAGTCCTGGTCC | |||||||||
| 51 | F:CCCCCTCTTCTTCTTCAACC | (CCAA)4 | 57.8 | 202 | 22 | 22 | 100.00 | 0.974 | Wang H. B. et al., 2013 |
| R:CAATAGAAAGCGCGTGACAA | |||||||||
| 53 | F:TCGAAGACAATCAGCACCTG | (ATG)7 | 57.8 | 233 | 18 | 17 | 94.44 | 0.961 | Wang H. B. et al., 2013 |
| R:TAAGTGTTCTTCCAGCGCCT | |||||||||
| 64 | F:GGCGATGGATGATGATGATT | (TTC)9 | 56.8 | 267 | 22 | 22 | 100.00 | 0.991 | Wang H. B. et al., 2013 |
| R:GAAAGAGGTGGATCGGATGA | |||||||||
| 86 | F:AAACCACCAAACCCATCAAA | (TGG)8 | 54.7 | 223 | 25 | 25 | 100.00 | 0.990 | Wang H. B. et al., 2013 |
| R:AACTTTGCCAGCATCGACTT | |||||||||
| 135 | F:CATTCCTACCCATCCCTCCT | (GTGGAG)4 | 58.8 | 100 | 31 | 31 | 100.00 | 0.993 | Wang H. B. et al., 2013 |
| R:CGCATGAGTGAGCCTAATGA | |||||||||
| 204 | F:TGAGCTTCATCCGCTTCTTT | (TGA)8 | 55.8 | 262 | 10 | 10 | 100.00 | 0.985 | Wang H. B. et al., 2013 |
| R:TGGTCGTATTCCGTCCATTT | |||||||||
| 219 | F:AAAAGGTTGTGAGTGGGTCG | (GGGAAG)4…(TGAGGG)4 | 57.8 | 228 | 26 | 25 | 96.15 | 0.976 | Wang H. B. et al., 2013 |
| R:CCTCGGTCGATAAATCTCCA | |||||||||
| 221 | F:AACCATGAATCCAGACACCC | (TCA)7 | 57.8 | 181 | 20 | 20 | 100.00 | 0.964 | Wang H. B. et al., 2013 |
| R:ACCAAGCCAGTCGAGTTTTG | |||||||||
| 235 | F:GCCCCAATTTATTCACTCCA | (AAC)6 | 57.8 | 257 | 30 | 30 | 100.00 | 0.979 | Wang H. B. et al., 2013 |
| R:GCTCTTCCTCGTAAGCATCG | |||||||||
| 262 | F:TCTGCCAGCTTTGGGTAACT | (CTTTTT)4 | 57.8 | 260 | 13 | 13 | 100.00 | 0.946 | Wang H. B. et al., 2013 |
| R:GTGCGCCTGTATTGACTTGA | |||||||||
| 270 | F:AGGTGGAAAATACTGTGCGG | (ATAGTA)4 | 57.8 | 139 | 19 | 19 | 100.00 | 0.966 | Wang H. B. et al., 2013 |
| R:TGTTTCTGCACCTCAACAGC | |||||||||
| 285 | F:CCGGTGTTCGGTATAAATGG | (GTG)7 | 56.8 | 116 | 11 | 11 | 100.00 | 0.965 | Wang H. B. et al., 2013 |
| R:ACAATTCGCTTCGGCTCTAA | |||||||||
| 312 | F:GGCCCAAGTTTGAGACAAAA | (AAG)7 | 56.8 | 219 | 36 | 36 | 100.00 | 0.985 | Wang H. B. et al., 2013 |
| R:TCGGTATAAGTGCACCACGA | |||||||||
| 313 | F:GGCGTTCTCTTCCATTTCAA | (GAA)7 | 56.8 | 253 | 37 | 37 | 100.00 | 0.984 | Wang H. B. et al., 2013 |
| R:GTTTTGGACCTTGCTTCTGC | |||||||||
| 320 | F:GGTCCTTCGTTTCATTTGGA | (TGG)7 | 57.8 | 235 | 21 | 21 | 100.00 | 0.973 | Wang H. B. et al., 2013 |
| R:CGGGGGTAGGAATAGAAAGC | |||||||||
| 327 | F:GAATGCAGCCTCAACAACAA | (TCAAAG)4 | 55.8 | 219 | 27 | 27 | 100.00 | 0.979 | Wang H. B. et al., 2013 |
| R:GAGCCGCCATTGTCATATTT | |||||||||
| 357 | F:ACCCAACCTGAACAAGATGC | (GGGTCA)4 | 58.8 | 252 | 24 | 24 | 100.00 | 0,990 | Wang H. B. et al., 2013 |
| R:ATACTGCTGCCACTGACCCT | |||||||||
| 581 | F:CCAATCCCAAACACTCCCTA | (CA)16 | 58.8 | 223 | 24 | 24 | 100.00 | 0.976 | Wang H. B. et al., 2013 |
| R:GCCGTTACCACTGCTCTTTC | |||||||||
| 984 | F:TCAAAACCCATCATCACCCT | (ACA)7 | 55.8 | 186 | 28 | 27 | 96.43 | 0.979 | Wang H. B. et al., 2013 |
| R:CGGCGTTTGTATCTTGGTTT | |||||||||
| 995 | F:TTGTTCCACGTGACGAGATT | (TGTTGG)4 | 56.9 | 244 | 16 | 16 | 100.00 | 0.961 | Wang H. B. et al., 2013 |
| R:CTCCCAAATGACCCATCATC | |||||||||
| 1036 | F:CTTTGGTAAGCGAAGGCTGT | (AATG)7 | 55.7 | 153 | 16 | 16 | 100.00 | 0.955 | Wang H. B. et al., 2013 |
| R:GCCATTTGTAAGCGGTTTGT | |||||||||
| 1187 | F:GAAAGCGATCATTGGGAAAA | (GAGAAG)4 | 54.7 | 112 | 19 | 19 | 100.00 | 0.963 | Wang H. B. et al., 2013 |
| R:TTACCCGTACATTCGGGATT | |||||||||
| 1424 | F:TAAAATCCATCCGTCCATCC | (CAA)7 | 57.8 | 278 | 26 | 26 | 100.00 | 0.975 | Wang H. B. et al., 2013 |
| R:CTTCCATATCTGCCAGTGGG | |||||||||
| 1428 | F:AACGCCCAAAACACCAACT | (AC)9 | 56.6 | 224 | 26 | 26 | 100.00 | 0.978 | Wang H. B. et al., 2013 |
| R:TAGAACCTTGTGCCCCCATA | |||||||||
| 1520 | F:AAATCACGGATCCCCTTCTT | (ATAGA)4 | 57.8 | 158 | 32 | 32 | 100.00 | 0.982 | Wang H. B. et al., 2013 |
| R:TTATCATCTTGGGGAGTGGC | |||||||||
| 1584 | F:CCTCCTCAAAACGACCATGT | (ACA)7 | 57.8 | 263 | 24 | 23 | 95.83 | 0.973 | Wang H. B. et al., 2013 |
| R:CGTCCCCATTACAATATCCG | |||||||||
| 1742 | F:AAGTGATAAGATGGGTGGCG | (TGG)7 | 57.8 | 166 | 28 | 28 | 100.00 | 0.974 | Wang H. B. et al., 2013 |
| R:GGTGGAGGCTCATTCAAATC | |||||||||
| 1762 | F:GCGTCAAATTACTGGTGGCT | (AAC)6 | 56.8 | 259 | 22 | 21 | 95.45 | 0.972 | Wang H. B. et al., 2013 |
| R:GTCTCATTTTCCGGCGATAA | |||||||||
| 1773 | F:CAAATGGGGTCGTTACGAAT | (CAT)6 | 55.8 | 215 | 17 | 17 | 100.00 | 0.965 | Wang H. B. et al., 2013 |
| R:AATCCCCGAATTCCCAATAG | |||||||||
| 1774 | F:TCACCACCACCACTGTCACT | (CACCGG)4 | 59.8 | 276 | 38 | 38 | 100.00 | 0.984 | Wang H. B. et al., 2013 |
| R:TGTGGGCTCTAGAGGTTTGG | |||||||||
| 1779 | F:AAAGTCCCCTTGCTTGTTCC | (AACC)5 | 57.8 | 148 | 25 | 25 | 100.00 | 0.975 | Wang H. B. et al., 2013 |
| R:CGACTCCATTTGATCCACCT | |||||||||
| Total | 1319 | 1306 | |||||||
| Average | 23.98 | 23.75 | 98.90% | 0.972 |