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. 2016 Jun 15;7:932. doi: 10.3389/fmicb.2016.00932

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Copyright © 2016 Yang, Zhang, Zhang, Zhou, Ma and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Product patterns of CcCel9A(A,D), CcCel9B(B) and CcCel48A(C) on cello-oligosaccharides. (A–C): G1-G6 (lanes 1-6) refer to the positions of standards (4 μg of each): glucose (G1), cellobiose (G2), cellotriose (G3), cellotetraose (G4), cellopentaose (G5), and cellohexose (G6), respectively. Reactions containing G2-G6 (0.4%) were incubated at 60°C for 16 h with 0.5 μmol enzyme. Samples loaded in lanes 7-11 were the hydrolysis products of G2 (1 μL), G3 (2 μL), G4 (2 μL), G5 (2 μL), and G6 (3 μL), respectively. (D): CcCel9A aliquots were incubated for 10, 30, and 120 min with cellotetraose (G4, lanes 1-3), cellopentaose (G5, lanes 4-6), and cellohexaose (G6, lanes 7-9). Lane 10, markers G1-G6 (from top down).