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. 2016 Jun 15;4:46. doi: 10.3389/fbioe.2016.00046

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Copyright © 2016 Albers and Offenhäusser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neuronal cell growth on the gateway between two adjacent structures after 14 days in vitro. (A) Cell growth between two CT1 structures. Neurons are stained against MAP2 (magenta) and NFH (orange). Scale bar: 50 μm. (B) Close-up of the gateway. The axons and the dendrites follow the base and overgrow the gateway without turning, whereas the axons from the lower structure sprout into the upper structure. Scale bar: 25 μm.