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. 2016 Jun 15;7:810. doi: 10.3389/fmicb.2016.00810

FIGURE 3.

FIGURE 3

Schematic representation of the tRNA cluster located between mfd and hybF in A. pleuropneumoniae isolates. Note that all sequences are shown in the forward orientation for simplicity. In the MIDG2331 genome, these sequences are on the complement strand. (A) The tRNA genes in isolates used as conjugal recipients are in the same order in (i) MIDG2465 (serovar 7), MIDG3217 (serovar 8), and MIDG3347 (serovar 12); and a different order in (ii) MIDG3376 (serovar 6). Note that all of the tRNA-Leu (TAA) genes have the sequence shown in Figure 1B. (B) Following conjugation, ICEApl1 integration resulted in loss of 3 tRNA genes, with all of the transconjugants (MIDG2465::ICEApl1, MIDG3217::ICEApl1, MIDG3347::ICEApl1, and MIDG3376::ICEApl1) showing identical sequences flanking the insertion (ICEApl1 shown boxed in blue). Note that the tRNA-Leu (TAA) gene shown in red has the altered bases of the attL DRs in Figure 1A. (C) In the 32 isolates with endogenous ICEApl1, the tRNA genes are found in three different orders in (i) MIDG2331 and 21 other isolates; (ii) 9 other isolates; and (iii) MIDG2648. Note the truncated element in MIDG2648 is shown as ICEApl1∗ (in purple text, boxed in purple). See Table 1 for details of specific isolates. Again, the tRNA-Leu (TAA) gene shown in red has the altered bases of the attL DRs in Figure 1A.