Table 1.

Clinical isolates of Actinobacillus pleuropneumoniae with ICEApl1.

Isolate ID	Serovar	Location of isolationa	Year of isolation	Length of ICEApl1b	5′ tRNAsc	Accession number for ICEApl1 sequence
MIDG2331	8	Thirsk	1995	56,083 bp	GLGLL	LN908249 (bases 1570419–1570505)d
MIDG2427	8	Aberdeen	1998	56,083 bp	GLGLL	KU551309
MIDG2648	8	Bury St Edmunds	2005	54,898 bp	GLGL	KU551310
MIDG2652	8	Thirsk	2005	56,083 bp	GLGLL	KU551311
MIDG2654	8	Winchester	2005	56,070 bp	GLGLL	KU551312
MIDG2657	8	Winchester	2005	56,083 bp	GLLGL	KU551313
MIDG2663	8	Thirsk	2005	56,083 bp	GLLGL	KU551314
MIDG2664	8	Bury St Edmunds	2005	56,083 bp	GLLGL	KU551315
MIDG3200	8	Thirsk	2006	56,083 bp	GLGLL	KU551316
MIDG3201	8	Bury St Edmunds	2006	56,083 bp	GLGLL	KU551317
MIDG3221	8	Langford	2006	56,083 bp	GLGLL	KU551318
MIDG3229	8	Thirsk	2007	56,083 bp	GLGLL	KU551320
MIDG3232	8	Thirsk	2007	56,083 bp	GLGLL	KU551321
MIDG3339	8	Winchester	2008	56,083 bp	GLGLL	KU551322
MIDG3344	8	Langford	2005	56,083 bp	GLLGL	KU551323
MIDG3346	8	Thirsk	2005	56,083 bp	GLLGL	KU551324
MIDG3349	8	Thirsk	2006	56,083 bp	GLLGL	KU551325
MIDG3357	8	Shrewsbury	2008	56,083 bp	GLGLL	KU551326
MIDG3368	8	Thirsk	2008	56,083 bp	GLGLL	KU551327
MIDG3370	8	Thirsk	2009	56,083 bp	GLLGL	KU551328
MIDG3371	8	Thirsk	2009	56,083 bp	GLLGL	KU551329
MIDG3372	8	Thirsk	2009	56,083 bp	GLLGL	KU551330
MIDG3378	8	Bury St Edmunds	2009	56,047 bp	GLGLL	KU551331
MIDG3381	8	Thirsk	2009	56,083 bp	GLGLL	KU551332
MIDG3386	8	Bury St Edmunds	2009	56,083 bp	GLGLL	KU551333
MIDG3388	8	Thirsk	2009	56,083 bp	GLGLL	KU551334
MIDG3389	8	Thirsk	2009	56,083 bp	GLGLL	KU551335
MIDG3395	8	Thirsk	2010	56, 011 bp	GLGLL	KU551336
MIDG3401	8	Bury St Edmunds	2011	56,083 bp	GLGLL	KU551337
MIDG3409	8	Bury St Edmunds	2011	56,083 bp	GLGLL	KU551338
MIDG3458	8	Cyprus	2011	56,012 bp	GLGLL	KU551339
MIDG3469	8	Thirsk	2012	56,083 bp	GLGLL	KU551340

^aAll isolates except MIDG2427 and MIDG3458 were cultured from pigs submitted to the then Veterinary Laboratories Agency (now Animal and Plant Health Agency) regional laboratories in the UK, as indicated.

^bThe length of each ICEApl1 sequence, calculated as the predicted circular form, is shown for each isolate. Size variation from that in MIDG2331 is indicated in bold. The sequence in MIDG2648 is missing 3 genes and has a truncated copy of the site-specific recombinase gene; whereas small deletions in the other sequences are all intergenic.

^cOrder of tRNA genes upstream of ICEApl1: G, tRNA-Gly (GCC), L, tRNA-Leu (TAA). Variation from the order seen in MIDG2331 is indicated in bold.

^dICEApl1 identified in MIDG2331 in previous study,⁴ all other ICEApl1 sequences were identified in this study.