Table 2.

Experimental data compared to calculated values determined by dynamic bonded cumomer modeling (mmol/L-cell/h).

Reaction	Exp. Flux (mmol/L-cell/h)	Calculated flux (mmol/L-cell/h)
Oxygen consumption MRO2	36	32
Glucose uptake	91.0	85
Pyruvate to lactate	155.0	150
Glutamine uptake	5.0	–a
Glutamate concentration	8.6 mM	9.2 mM

^aThe flux was not estimated, as glutamine is consumed in numerous reactions we did not study here. This research was originally published in Journal of Biological Chemistry. Shestov et al. (14). The American Society for Biochemistry and Molecular Biology.