Table 4.
Changes in gene expression following treatment of DLCL2 cells with rapamycin.a
| Entrez ID | Gene Symbol | Enzyme name | EC number | Fold change | P value | Pathway Affected |
|---|---|---|---|---|---|---|
| Upregulated | ||||||
| 5106 | PCK2 | Phosphoenolpyruvate carboxykinase 2 (mit) | EC 4.1.1.32 | 1.51 | 0.000434 | Pyruvate cycling |
| Downregulated | ||||||
| 3099 | HK2 | Hexokinase 2 | EC 2.7.1.1 | −1.78 | 0.00515 | Glycolysis |
| 57546 | PDP2 | Pyruvate DH phosphatase subunit 2 | EC3.1.3.43 | −1.54 | 0.0150 | PDH, TCA |
| 3419 | IDH3A | Isocitrate DH 3 (NAD +) alpha (mito) | EC1.1.1.41 | −1.60 | 0.000673 | TCA Cycle |
| 5471 | PPAT | Amidophosphoribosyl transferase | EC2.4.2.14 | −1.73 | 0.0163 | Glutaminolysisb |
| 31 | ACACA | Acetyl-CoA carboxylase alpha | EC6.3.4.14 | −1.80 | 0.00518 | Fatty acids |
| 2180 | ACSL1 | Acyl-CoA synthetase long-chain member 1 | EC 6.2.1.3 | −1.52 | 0.0161 | Fatty acids |
| 23171 | GPD1L | Glycerol-3-phosphate dehydrogenase 1-like | EC 1.1.1.8 | −1.56 | 0.00446 | Glycerophospholipid |
| 22934 | RPIA | Ribose 5-phosphate isomerase A | EC 5.3.1.6 | −1.58 | 0.00451 | Pentose phosphate |
| 1717 | DHCR7 | 7-dehydrocholesterol reductase | EC1.3.1.21 | −1.72 | 0.00363 | Cholesterol |
| 6307 | MSMO1 | Methylsterol monooxygenase 1 | EC1.14.13.72 | −1.74 | 0.0165 | Cholesterol |
| 1723 | DHODH | Dihydroorotate dehydrogenase | EC 1.3.5.2 | −1.51 | 0.000190 | Pyrimidine metabolism |
| 5198 | PFAS | Phosphoribosylformylglycinamidine synthase | EC 6.3.5.3 | −1.51 | 0.00120 | Purine metabolism |
aData were compiled from KEGG database. Gene expression of a large number of enzymes demonstrated statistically significant changes, but only one or two representative enzymes are listed for pathways showing changes greater than 50%.
bThis is a new link for glutamine to glutamate interconversion through operation of enzyme 5-phospho-β-d-ribosylamine:diphosphate phospho-α-d-ribosyl-transferase (glutamate-amidation reaction).