Table 1.
Major characteristics of DGE libraries and tag mapping to reference sequences database.
MP | HP | PT | ||||
---|---|---|---|---|---|---|
Distinct tag | Total tag number | Distinct tag | Total tag number | Distinct tag | Total tag number | |
Raw data | 346,420 | 4,746,710 | 286,685 | 3,188,914 | 212,377 | 2,637,062 |
Tags containing N | 39,861 | 57,487 | 26,579 | 37,599 | 23,156 | 32,326 |
Adaptors | 0 | 0 | 0 | 0 | 0 | 0 |
Tag CopyNum < 2 | 180,056 | 180,056 | 161,267 | 161,267 | 98,679 | 98,679 |
Clean tag | 126,503 | 4,509,167 | 98,839 | 2,990,048 | 90,542 | 2,506,057 |
CopyNum > = 2 | 126,503 | 4,509,167 | 98,839 | 2,990,048 | 90,542 | 2,506,057 |
CopyNum >5 | 51,791 | 4,297,810 | 36,024 | 2,816,071 | 34,573 | 2,342,951 |
CopyNum >10 | 33,568 | 4,159,257 | 22,702 | 2,715,125 | 20,722 | 2,238,622 |
CopyNum >20 | 20,945 | 3,974,730 | 13,751 | 2,584,585 | 12,131 | 2,113,464 |
CopyNum >50 | 10,372 | 3,637,058 | 6569 | 2,356,948 | 5621 | 1,907,976 |
CopyNum >100 | 5733 | 3,309,786 | 3719 | 2,155,101 | 3012 | 1,724,226 |
Tag mapping | ||||||
All mapping | 59,339 | 2,340,206 | 41,979 | 1,381,560 | 39,734 | 1,215,984 |
Unambiguous mapping | 47,294 | 1,659,654 | 32,998 | 968,992 | 31,259 | 824,530 |
Mapping to genome | 23,473 | 990,689 | 17,076 | 705,316 | 15,666 | 493,927 |
Unknown tag | 24,469 | 561,809 | 23,781 | 442,972 | 21,682 | 463,703 |
All Mapping represents all Tags Mapping to Gene (Sense and Anti-Senese). Unambiguous Mapping represents unambiguous Tag Mapping to Gene (Sense and Anti-Sense). Mapping to genome represents the clean tags that can not be mapped to mRNA, mitochondria and chloroplast are mapped to the whole genome, providing zones that can be uniquely mapped by those tags.