FIGURE 7.

Co-administration of GlcN6SO₄ increases susceptibility of wild-type E. coli towards Nva-FMDP. (A) Disk diffusion assay to address the combined effect of Nva-FMDP and GlcN6SO₄ on E. coli. Filter disks containing 50 μg Nva-FMDP and various GlcN6SO₄ amounts as indicated were applied to MacConkey lactose plates that were seeded with E. coli wild-type strain Z854. An exemplary experiment is shown at the top. A diagram depicting the results of three independent experiments is shown below. (B) Effect of GlcN6SO₄ on susceptibility of E. coli to Nva-FMDP in LB liquid medium. Serial dilutions of Nva-FMDP or GlcN6SO₄ or a mixture of both compounds were prepared in LB medium and subsequently inoculated with 5 × 10⁵ CFU/ml of E. coli strain Z854. Cell densities were recorded following 12 h incubation.