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. 2016 May 26;98(6):1101–1113. doi: 10.1016/j.ajhg.2016.03.028

Figure 2.

Figure 2

Evaluation of the Efficiency and Specificity of AAV Transduction

(A) Comparative transduction efficiency and specificity of rAAV2/1 and rAAV2/9 carrying a CMV-eGFP expression construct delivered to wild-type murine cochlea at P0–P2 via a trans-RWM approach. rAAV2/1 transduces IHCs, OHCs, spiral ganglion cells (arrow), and inner sulcus cells (arrowhead) in the osseous spiral lamina (OSL). Overlapping MYO7A and eGFP localization represents positive hair cell transduction. Note that compared to rAAV2/1, rAAV2/9 shows specific IHC transduction. Scale bars represent 50 μm.

(B) The efficiency of viral transuction in IHCs was assessed in 400 μm segments in the apical and basal turns (rAAV2/1 [gray] and rAAV2/9 [black]). Note the high (∼74%) rAAV2/9 IHC transduction in the apical turn. Error bars represent the SD (n = 3). ∗p < 0.05, ∗∗p < 0.005.