Figure 4.

The Risk Allele of SNP rs2494737 Enhances AKT1 Promoter Activity

Luciferase reporter assays following transient transfection of Ishikawa endometrial cancer cell lines. The putative regulatory element (PRE) containing the major SNP alleles were cloned downstream of target gene promoter-driven luciferase constructs. AKT1 can and AKT1 alt denote a canonical and alternative AKT1 promoter (prom) region, respectively. Minor (risk-increasing) SNP alleles were engineered into the constructs and are designated by the rs ID of the corresponding SNP. Haplotype denotes a construct that contains the minor alleles of rs2498796 and rs2494737. Error bars denote 95% confidence intervals from three independent experiments performed in duplicate. P values were determined by 2-way ANOVA followed by Dunnett’s multiple comparisons test (^∗∗p < 0.01, ^∗∗∗∗p < 0.0001).