Table 3.
FAD, FMN, and Riboflavin Quantification in Cellular and Mitochondrial Fibroblast Samples from Control and Affected Individuals
| ID | FAD | FMN | Riboflavin | FAD/FMN Ratio | |
|---|---|---|---|---|---|
| Cellular flavin content (pmol/mg) | C1 | 111.7 ± 2.8 | 13.3 ± 1.4 | 2.6 ± 0.5 | 8.5 ± 0.7 |
| C2 | 171.2 ± 18.8 | 12.4 ± 0.3 | 3.1 ± 0.1 | 13.8 ± 1.9 | |
| C3 | 105.8 ± 6.9 | 13.0 ± 0.0 | 2.1 ± 0.6 | 8.2 ± 0.5 | |
| S1a | 82.3 ± 8.6 | 11.7 ± 0.8 | 2.1 ± 0.8 | 7.0 ± 0.2 | |
| S2 | 84.3 ± 3.1 | 11.5 ± 0.6 | 2.4 ± 0.9 | 7.3 ± 0.1 | |
| S4a | 111.4 ± 6.6 | 15.8 ± 0.4 | 2.5 ± 0.5 | 7.0 ± 0.6 | |
| Mitochondrial flavin content (pmol/mg) | C1 | 2,093 | 3.0 ± 0.1 | NT | 697.7 ± 24.0 |
| C2 | 1,940 | 3.2 | NT | 606.3 | |
| C3 | 1,895 | 3.2 | NT | 592.2 | |
| S4a | 1,310 ± 70∗∗ | 2.0 ± 0.7 | NT | 655.0 ± 195.7 |
For cellular flavin content, data represent the mean ± SD of two independent cell lysates. NT, not tested. ∗∗p < 0.01.