Table 4.

Kinetic Properties of 6His-p.Ser495del, 6His-p.Arg530Cys, and WT FADS2

Forward Reaction	6His-p.Arg530Cys	6His-p.Ser495del	WT
Km FMN (μM)	0.39 ± 0.07	0.98 ± 0.23∗∗∗	0.32 ± 0.10
Km ATP (μM)	4.63 ± 1.00∗	8.02 ± 2.23	8.10 ± 1.37
Vmax (nmol·min−1·mg−1)	7.9 ± 3.7∗∗∗	10.2 ± 1.6∗∗	43.3 ± 12.2
Mg2+50 (mM)	0.04 ± 0.02∗	0.09 ± 0.01	0.07 ± 0.01

Data represent the mean ± SD of three experiments. ^∗p < 0.05; ^∗∗p < 0.01, ^∗∗∗p < 0.001.