Table 4.
Forward Reaction | 6His-p.Arg530Cys | 6His-p.Ser495del | WT |
---|---|---|---|
Km FMN (μM) | 0.39 ± 0.07 | 0.98 ± 0.23∗∗∗ | 0.32 ± 0.10 |
Km ATP (μM) | 4.63 ± 1.00∗ | 8.02 ± 2.23 | 8.10 ± 1.37 |
Vmax (nmol·min−1·mg−1) | 7.9 ± 3.7∗∗∗ | 10.2 ± 1.6∗∗ | 43.3 ± 12.2 |
Mg2+50 (mM) | 0.04 ± 0.02∗ | 0.09 ± 0.01 | 0.07 ± 0.01 |
Data represent the mean ± SD of three experiments. ∗p < 0.05; ∗∗p < 0.01, ∗∗∗p < 0.001.