Figure 4.

The Effect of PRDM6 Amino Acid Substitutions on MYH11 Cellular Levels

Immunostaining demonstrates the localization of PRDM6 (red) in relationship to the nucleus (blue) and the staining for MYH11 in human aortic smooth muscle cells. Cells infected with wild-type PRDM6, PRDM6 p.Arg549Gln (p.R549Q), and PRDM6 p.Cys263Ser (p.C263S) are shown in the top, middle, and bottom rows, respectively. Wild-type PRDM6 suppressed MYH11. Substitution p.Arg549Gln was largely retained in the cytoplasm, whereas p.Cys263Ser was mainly expressed in the nucleus. In smooth muscle cells overexpressing DNA encoding p.Arg549Gln and p.Cys263Ser, MYH11 staining was comparable to that in non-transfected cells. Dotted lines delineate transfected cells. Scale bars represent 28 μm.