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. 2016 Apr 21;173(13):2147–2161. doi: 10.1111/bph.13484

Table 1.

Primer sequences, annealing temperatures and PCR product length

Gene Sequence Annealing temperature, °C Product length, bp
D13Mit76 marker F5′‐atgcacctgtctaaatgtgtgc‐3′ 60 109
R5′‐agagggactgtgggactgtg‐3′
D13Mit78 marker F5′‐acagcacgggtttatcatcc‐3′ 60 229
R5′‐tatgcctgccaggcttctat‐3′
BDNF F5′‐ tagcaaaaagagaattggctg −3′ 59 255
R5′‐ tttcaggtcatggatatgtcc −3′
TrkB F5′‐cattcactgtgagaggcaacc‐3′ 63 175
F5′‐atcagggtgtagtctccgttatt‐3′
p75 F5′‐ acaacacccagcacccagga −3′ 62 171
R5′‐cacaaccacagcagccaaga −3′
MAO‐A F5′‐aatgaggatgttaaatgggtagatgttggt‐3′ 62 138
R5′‐cttgacatattcaactagacgctc‐3′
5‐HT 1A receptor F5′‐gactgccaccctctgccctatatc‐3′ 62 200
R5′‐tcagcaaggcaaacaattccag‐3′
5‐HT 2A receptor F5′‐agaagccaccttgtgtgtga‐3′ 61 169
R5′‐ttgctcattgctgatggact‐3′
Tph2 F5′‐cattcctcgcacaattccagtcg‐3′ 61 239
R5′‐agtctacatccatcccaactgctg‐3′
Slc6a4 F5′‐aagccccaccttgactcctcc‐3′ 57 198
R5′‐ctccttcctctcctcacatatcc‐3′
Polr2a F5′‐gttgtcgggcagcagaatgtag‐3′ 63 188
R5′‐tcaatgagaccttctcgtcctcc‐3′