Skip to main content
. 2012 Jan 31;2012:706545. doi: 10.5402/2012/706545

Figure 4.

Figure 4

Blocking EGFR signaling in Caco2 cells leads to reduction in PS74 and PS432 levels. Caco2 cells were treated with the EGFR ligand binding blocking antibody sc-120 for 4 hours, 6 hours, and 8 hours (n = 6 per treatment group). (a) Western blotting and band intensity quantification was performed on the cell lysates with antibodies against total K8, PS24, PS74, and PS432. Levels of PS74 and PS432 were significantly reduced 6 and 8 hours after sc-120 treatment, P < 0.0001. As expected, levels of PS24 remained unchanged by treatment with sc-120, P = 0.73. (b) Representative western blots: B1, PS432; B2, PS74; B3, PS24. The lower bands in each case are the β actin loading controls.