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. 2012 Jan 23;2012:823875. doi: 10.5402/2012/823875

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Copyright © 2012 Sara Iozzi et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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DKK1 inhibits Wnt3a-mediated β-catenin translocation. (A) Immunostaining of β-catenin in L-cells following stimulation with Wnt3a CM, either without DKK1 or in the presence of increasing volumes of DKK1 CM. Panel (a), unstimulated cells; panel (b), Wnt3a-stimulated cells; panels (c–f), Wnt3a-stimulated cells in presence of DKK1 CM dilutions. Cell nuclei counterstaining with Hoechst 33342 dye are shown for each panel. (B) Histogram representation of the nuclear/cytoplasmic intensity ratios corresponding to the cells shown in (A).