Subject area	Biology
More specific subject area	RNA-seq transcriptome data of barley (Hordeum vulgare)
Type of data	Tables and figures
How data was acquired	Sequencing on Illumina HiSeq 2500 Sequencing System
Data format	Processed, analyzed
Experimental factors	Samples were exposed to severe drought stress and subsequently re-watered
Experimental features	RNA was extracted using RNAqueous kit and purified on magnetic beads. Sequencing libraries were prepared using the TruSeq Stranded mRNA kit from Illumina and quantified using the Kapa Library Quantification kit. Libraries were sequenced on HiSeq 2500 Illumina platform.
Data source location	Palacký University, Olomouc, Czech Republic
Data accessibility	Data are within this article