Fig. 1.

Assay of total GSH and rGSH in brain lysates from healthy, AD, and HIV-1 positive subjects. Frontal cortex specimen was homogenized and extracted with PBS buffer. GSH assay was performed using a colorimetric assay kit from Arbor Assays. Data represent means ± SE from 5 healthy individuals, 5 individuals with AD and 10 HIV-1-positive subjects, *p < 0.05, **p < 0.05. HIV-1-positive subjects were sub-divided by CD4 + T-cell counts: low CD4 + T-cell < 200 cells/mm³ and high CD4 + T-cell > 200 cells/mm³. Panel A: total GSH were significantly lower from HIV-1-positive subjects with low CD4 + T-cells and high CD4 + T-cells compared to healthy subjects. Data represents means ± SE from 5 healthy subjects, 5 AD, and 5 HIV-1-positive individuals *p < 0.05, **p < 0.05. No statistical significance was shown between healthy and AD patients. Panel B: there was a significant decrease in the levels of rGSH in HIV-1-positive subjects with low CD4 + T-cells and high CD4 + T-cells compared to healthy subjects. Data represents means ± SE from 5 healthy subjects, 5 AD, and 5 HIV-1-positive individuals *p < 0.05, **p < 0.05.