Fig. 2.

Western blot analysis of a GSH de novo synthesis enzyme, GCLC, in brain tissue from healthy subjects, AD and HIV-1-positive subjects. Data represent means ± SE,*p < 0.05. Panels A and B: Western blot results were corrected for GAPDH and reported as relative intensity compared to GAPDH. Levels of GCLC were significantly lower from HIV-1-positive subjects compared to healthy and AD subjects (B; n = 5 healthy, n = 4 AD, n = 5 HIV-1-positive). HIV-1-positive subjects with low CD4 + T-cells were observed to have significant decrease in the levels of GCLC compared to healthy subjects. No significant difference was observed with HIV-1-positive subjects with high CD4 + T-cells compared to healthy subjects.