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. 2016 Apr 18;185(1):22–32. doi: 10.1111/cei.12791

Figure 2.

Figure 2

Deletion of CXCR5 in a murine lupus model reduces the humoral immune response and increases life expectancy. (a) Microscope photos show frozen spleen sections from 15–16‐week‐old B6/lpr (left) and B6/lpr CXCR5–/– (right) mice that were stained with anti‐CD3‐allophycocyanin (APC) antibody (red) for T cells and biotinylated peanut agglutinin (PNA)/streptavidin DyLight‐488 (green) for germinal centre (GC) cells. The slides were imaged with a ×10 objective using a fluorescence microscope. Statistical analysis of frozen spleen sections stained with anti‐CD3‐allophycocyanin (APC) antibody and biotinylated PNA/streptavidin DyLight‐488. The numbers of GCs per mm2 spleen were counted in 12–16‐week‐old B6/lpr and B6/lpr CXCR5–/– mice. At least four mice per group were analysed. (b) Percentages of splenic CD138+ cells from 10–11‐week‐old B6/lpr and B6/lpr CXCR5–/– mice assessed by flow cytometry (at least four mice per group). (c) Total immunoglobulin (Ig)G enzyme‐linked immunosorbent assay (ELISA) of sera from aged (> 45 weeks) B6/lpr and B6/lpr CXCR5–/– mice. Data indicate mean values of µg/ml IgG in serum ± standard error of the mean (s.e.m.) of at least eight mice per group. (d) Kaplan–Meier survival plots were used to assess the life expectancy rates of B6/lpr and B6/lpr CXCR5–/– mice; 69 B6/lpr mice and 134 B6/lpr CXCR5–/– mice were observed.