Figure 4.

Cholangiocarcinoma (CC)‐associated miRNAs in primary sclerosing cholangitis (PSC). (a) P‐value relative to box‐plot (b) and odds ratio of CC‐associated miRNA in PSC. (b) miRNA levels. The box‐plot shows the minimum, median and maximum of miRNA levels. A standard t‐test was performed and miRNA were considered represented differently if absolute log₁₀(RQ) > 1 and P‐value < 0·05 (P‐values are shown in the table). Two miRNAs were identified: miR‐222 and miR‐483‐5p. (c) Receiver operating characteristic (ROC) curve analysis of CC‐associated miRNAs and ROC curve analysis combined with the altered miRNAs. Fraction of true positive results (sensitivity) and false positive results (1‐specificity) for levels of miRNA are shown. ROC curves were obtained using logistic regression, and the area under the curve (AUC) was calculated using the Epicalc package. AUC was considered significant if > 0·70.