Figure 2. Amino acid sequences alignment and structure comparison between murine and humanized antibody.

(a) VH sequence alignment and (b) VL sequence alignment. MMGZ01_VH and MMGZ01_VL were named for VH and VL regions of MMGZ01 murine antibody. Z27506 and J00256 found in V and J genes were chosen as human FRs donors for the humanized VH. Similarly, X63399 and J00242 were chosen as human FRs donors for the humanized VL. VH_g and VL_g were VH and VL regions of CDR grafted antibody. VH₃ and VL₂ were VH and VL regions of the final version of humanized antibody. The residues colored red were back mutations in the final version. * represents residues that are identical to the corresponding residues of MMGZ01. CDR1-2 regions of VH are marked orange and CDR3 region of VH are marked red. CDR1-3 regions of VL are marked purple. CDR regions were identified with the Kabat numbering scheme¹⁷. (c) Superposed structure of MMGZ01 Fv and H₃L₂ Fv. The calculated structural RMSD was 0.824 Å.