Table 2. Canonical residues in FRs of VH & VL for back mutation.
Position | Residues | Interface core | Loop foundation | Loop interaction |
---|---|---|---|---|
LFR1 | I4 | 0 | 1 | 1 |
LFR2 | P47 | 1 | 1 | 0 |
LFR2 | W48 | 0 | 1 | 0 |
LFR2 | Y50 | 1 | 1 | 1 |
LFR3 | Y72 | 0 | 1 | 1 |
HFR1 | Y27 | 0 | 1 | 0 |
HFR2 | I37 | 1 | 1 | 1 |
HFR2 | K38 | 1 | 1 | 0 |
HFR2 | I48 | 0 | 1 | 1 |
HFR3 | A68 | 0 | 1 | 0 |
HFR3 | L70 | 0 | 1 | 1 |
HFR3 | G98 | 0 | 1 | 0 |
1 represents yes and 0 represents no, residues shown are original murine ones.