Figure 2. An overlay of the active sites from wild-type avLOX (grey) and the Y215F variant (colored by atom type) is shown.

Hydrogen bonding in the wild-type structure is shown (yellow dashed line) including the hydrogen bond lost in the mutant (red dashed line). A subtle repositioning of the pyruvate and the phenylalanine side chain create a new hydrophobic contact in the mutant. This replaces a hydrogen bond with poor geometry between the tyrosine and pyruvate keto group.