Table 2. Mean and standard error of the calculated glycosylation traits for the time points used in the statistical analysis.
trim1* |
trim3 |
6wkpp |
26wkpp |
n | trim1 vs trim3 | trim3 vs 6wkpp | trim3 vs 26wkpp | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
% | SE | % | SE | % | SE | % | SE | ||||||
Asn 144 | Sialylation | 61.39 | 0.96 | 63.12 | 0.90 | 59.08 | 1.05 | 59.04 | 0.80 | 25 | ns | 0.0002 | 0.0002 |
Bisection | 25.47 | 0.72 | 27.20 | 0.90 | 28.47 | 0.88 | 26.85 | 0.72 | 25 | 0.0119 | ns | ns | |
Asn 340 | Sialylation | 95.18 | 0.17 | 95.35 | 0.16 | 95.40 | 0.19 | 95.22 | 0.26 | 23 | ns | ns | ns |
Bisection | 52.24 | 1.12 | 54.67 | 1.07 | 57.98 | 0.99 | 54.88 | 1.24 | 23 | 0.0072 | 0.0020 | ns | |
Asn340 (truncated) | Galactosylation | 99.84 | 0.01 | 99.83 | 0.01 | 99.82 | 0.01 | 99.84 | 0.01 | 25 | ns | ns | ns |
Sialylation | 89.56 | 0.21 | 89.12 | 0.19 | 89.26 | 0.23 | 89.25 | 0.27 | 25 | ns | ns | ns | |
Fucosylation | 92.94 | 0.34 | 92.26 | 0.36 | 92.90 | 0.35 | 93.38 | 0.26 | 25 | 0.0007 | 0.0014 | 0.0005 | |
Bisection | 51.76 | 1.04 | 53.87 | 1.00 | 58.45 | 1.03 | 55.36 | 0.94 | 25 | 0.0001 | 0.0000 | ns | |
Triantennary | 5.46 | 0.26 | 6.10 | 0.28 | 5.40 | 0.29 | 5.24 | 0.25 | 25 | 0.0017 | 0.0009 | 0.0080 | |
# | SE | # | SE | # | SE | # | SE | ||||||
O-glycosylation | Number of GalNAcs | 4.81 | 0.01 | 4.81 | 0.01 | 4.82 | 0.01 | 4.81 | 0.01 | 27 | ns | ns | ns |
Number of Gal | 3.96 | 0.02 | 3.98 | 0.02 | 3.99 | 0.01 | 3.99 | 0.01 | 27 | 0.0095 | ns | ns | |
Number of SA | 3.03 | 0.03 | 3.02 | 0.03 | 3.04 | 0.02 | 3.05 | 0.03 | 27 | ns | ns | ns | |
SA per Gal | 0.77 | 0.01 | 0.76 | 0.01 | 0.76 | 0.01 | 0.77 | 0.01 | 27 | ns | ns | ns | |
Gal per GalNAc | 0.82 | 0.004 | 0.83 | 0.003 | 0.83 | 0.003 | 0.83 | 0.003 | 27 | 0.0109 | ns | ns | |
% | SE | % | SE | % | SE | % | SE | ||||||
% with more GalNAc than Gal | 61.36 | 0.94 | 60.27 | 0.86 | 59.99 | 0.76 | 59.81 | 0.79 | 27 | ns | ns | ns | |
% with more SA than Gal | 6.49 | 0.43 | 6.79 | 0.36 | 6.69 | 0.30 | 6.59 | 0.31 | 27 | ns | ns | ns |
In addition the number of paired samples for the Wilcoxon signed-rank test are depicted, as well as the resulting p-values. P < 0.0167 was considered as statistically significant after Bonferroni correction.
*Abbreviations: trim = trimester; wkpp = weeks postpartum; GalNAc = N-acetylgalactosamine; Gal = galactose; SA = N-acetylneuraminic acid.