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. 2016 Jun 15;6:27991. doi: 10.1038/srep27991

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Copyright © 2016, Macmillan Publishers Limited

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(A) DDX4 comparison in human and mouse. The DDX4^C25 antibody used for the isolation of the OSCs was raised against the C-terminus (in red) of H.s DDX4. In M.m this sequence shares 92% identity. (B,C) DDX4^C25 immunostaining in permeabilized (B) and non-permeabilized (C) fully-grown oocytes and ovarian somatic cells (a mixture of stroma and granulosa cells). Staining was only observed in the cytoplasm of permeabilized oocytes. It total we analysed 70 oocytes and 250 somatic cells taken from 14 ovaries. Scale bar: 20 μm.