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. 2016 Jun 15;6:27897. doi: 10.1038/srep27897

Table 2. Precision and bias of potency metrics in gain-loss models.

Data Set Parameters Estimator
True |RMAX| True AC50(G) True AC50(L) True PODWES True Peak AC50 PODWES
5% error (15% Detection Limit)
25 0.001 0.1 3.23e-03 20.5 2.41 (+2.71) 1.44 (+0.12)
25 0.001 10 3.17e-03 24.5 7.48 (+2.03) 1.32 (+0.11)
25 0.1 10 0.36 20.5 3.28 (−0.82) 1.37 (−0.07)
50 0.001 0.1 1.43e-03 40.95 0.85 (+2.51) 1.03 (−0.04)
50 0.001 10 1.35e-03 49.0 6.42 (+1.95) 1.03 (−0.03)
50 0.1 10 0.18 40.95 0.88 (−0.51) 1.14 (−0.27)
100 0.001 0.001 5.80e-04 24.3 1.32 (+1.15) 0.86 (+0.05)
100 0.001 0.1 4.27e-04 81.9 0.37 (+2.48) 1.07 (+0.05)
100 0.001 10 4.19e-04 98.0 6.44 (+1.98) 1.07 (+0.06)
100 0.1 0.1 0.08 25.0 19.78 (−0.29) 0.95 (−0.20)
100 0.1 10 0.07 81.9 0.37 (−0.48) 1.21 (−0.16)
100 10 10 9.47 24.3 1.36 (−1.17) 0.94 (−0.42)
10% error (30% Detection Limit)
50 0.001 0.1 2.55e-03 40.95 2.60 (+2.72) 1.44 (+0.17)
50 0.001 10 2.89e-03 49.0 7.33 (+1.96) 1.32 (+0.12)
50 0.1 10 0.31 40.95 3.47 (−0.80) 1.36 (−0.04)
100 0.001 0.1 1.37e-03 81.9 0.87 (+2.51) 1.00 (−0.06)
100 0.001 10 1.25e-03 98.0 6.42 (+1.95) 1.00 (−0.03)
100 0.1 10 0.16 81.9 0.87 (−0.51) 0.94 (−0.32)

Equation (2) in the Methods was used to simulate data sets with the specified error. Log10Precision is shown with log10Bias in parentheses. Only data sets with true responses above the assay detection limit were evaluated.