Figure 2. Effect of the expression of SLC41A3 on the change of the intracellular free magnesium concentration (Δ[Mg²⁺]_i) in HEK293 cells.

(a) Tetracycline-induced (+tet) and uninduced (−tet) cells of the SLC41A3 overexpressing cell lines A3st (stable A3 expression) and Clone2 (transient A3 expression) were loaded with Mg²⁺, and changes of [Mg²⁺]_i after 1,200 s in completely Mg²⁺-free solution were determined. Wild-type HEK293 cells (HEK WT) served as a control. Values are given as means ± SEM. Number of measurements: N_{A3 −tet} = 17; N_{A3 +tet} = 12; N_{Cl2/A3 −tet} = 10; N_{Cl2/A3 +tet} = 10; N_{HEK WT} = 6. ^a,bColumns with different letters differ significantly in pairwise comparisons. (P < 0.05). (b) Representative original recordings of [Mg²⁺]_i changes of Mg²⁺ -preloaded cells measured in completely Mg²⁺-free external buffer solution.